Positive Feedback Research Articles

Assessing CESM2 Clouds and Their Response to Climate Change Using Cloud Regimes

Abstract The Community Earth System Model, version 2 (CESM2), has a very high climate sensitivity driven by strong positive cloud feedbacks. To evaluate the simulated clouds in the present climate and characterize their response with climate warming, a clustering approach is applied to three independent satellite cloud products and a set of coupled climate simulations. Using k-means clustering with a Wasserstein distance cost function, a set of typical cloud configurations is derived for the satellite cloud products. Using satellite simulator output, the model clouds are classified into the observed cloud regimes in both current and future climates. The model qualitatively reproduces the observed cloud configurations in the historical simulation using the same time period as the satellite observations, but it struggles to capture the observed heterogeneity of clouds which leads to an overestimation of the frequency of a few preferred cloud regimes. This problem is especially apparent for boundary layer clouds. Those low-level cloud regimes also account for much of the climate response in the late twenty-first century in four shared socioeconomic pathway simulations. The model reduces the frequency of occurrence of these low-cloud regimes, especially in tropical regions under large-scale subsidence, in favor of regimes that have weaker cloud radiative effects.

Enzymatic Reaction-Coupled, Cooperative Supramolecular Polymerization.

Nature employs sophisticated mechanisms to precisely regulate self-assembly and functions within biological systems, exemplified by the formation of cytoskeletal filaments. Various enzymatic reactions and auxiliary proteins couple with the self-assembly process, meticulously regulating the length and functions of resulting macromolecular structures. In this context, we present a bioinspired, reaction-coupled approach for the controlled supramolecular polymerization in synthetic systems. To achieve this, we employ an enzymatic reaction that interfaces with the adenosine triphosphate (ATP)-templated supramolecular polymerization of naphthalene diimide monomers (NSG). Notably, the enzymatic production of ATP (template) plays a pivotal role in facilitating reaction-controlled, cooperative growth of the NSG monomers. This growth process, in turn, provides positive feedback to the enzymatic production of ATP, creating an ideal reaction-coupled assembly process. The success of this approach is further evident in the living-growth characteristic observed during seeding experiments, marking this method as the pioneering instance where reaction-coupled self-assembly precisely controls the growth kinetics and structural aspects of supramolecular polymers in a predictive manner, akin to biological systems.

CYTOR-NFAT1 feedback loop regulates epithelial-mesenchymal transition of retinal pigment epithelial cells.

Epithelial mesenchymal transition (EMT) occurring in retinal pigment epithelial cells (RPE) is a crucial mechanism that contributes to the development of age-related macular degeneration (AMD), a pivotal factor leading topermanent vision impairment. Long non-coding RNAs (lncRNAs) have emerged as critical regulators orchestrating EMT in RPE cells. In this study, we explored the function of the lncRNA CYTOR (cytoskeleton regulator RNA) in EMT of RPE cells and its underlying mechanisms. Through weighted correlation network analysis, we identified CYTOR as an EMT-related lncRNA associated with AMD. Experimental validation revealed that CYTOR orchestrates TGF-β1-induced EMT, as well as proliferation and migration of ARPE-19 cells. Further investigation demonstrated the involvement of CYTOR in regulating the WNT5A/NFAT1 pathway and NFAT1 intranuclear translocation in the ARPE-19 cell EMT model. Mechanistically, CHIP, EMSA and dual luciferase reporter assays confirmed NFAT1's direct binding to CYTOR's promoter, promoting transcription. Reciprocally, CYTOR overexpression promoted NFAT1 expression, while NFAT1 overexpression increased CYTOR transcription. These findings highlight a mutual promotion between CYTOR and NFAT1, forming a positive feedback loop that triggers the EMT phenotype in ARPE-19 cells. These discoveries provide valuable insights into the molecular mechanisms of EMT and its association with AMD, offering potential avenues for targeted therapies in EMT-related conditions, including AMD.

HO-1-induced autophagy establishes a HO-1-p62-Nrf2 positive feedback loop to reduce gut permeability in cholestatic liver disease.

The gut-liver axis disruption is a unified pathogenetic principle of cholestatic liver disease (CSLD). Increased gut permeability is the leading cause of gut-liver axis disruption. HO-1 is capable of protecting against gut-liver axis injury. However, it has rarely been reported whether autophagy is involved in HO-1 protecting gut-liver barrier integrity and the underlying mechanism. Mice underwent bile duct ligation (BDL) was established as CSLD model in vivo. Caco-2 cells with LPS treatment was established as in vitro cell model. Immunofluorescence, western blot and transepithelial electrical resistance (TER) assay were used to observe epithelial tight junction (TJ) and autophagy. Liver injury and fibrosis were evaluated as well through H&E staining, masson staining, sirius red staining and ELISA. Our study demonstrated that the epithelial TJ and TER were notably reduced both in BDL mice and in LPS treated intestinal epithelial cells. Increased HO-1 expression could significantly induce intestinal epithelial cell autophagy. Additionally, this increased autophagy level reversed the reduction effects of BDL or LPS on epithelial TJ and TER in vivo and in vitro, therefore decreased transaminase level in serum and relieved liver fibrosis in BDL mice. Besides, increased autophagy level in turn upregulated the expression of HO-1 by p62 degradation of Keap1 and subsequent activation of Nrf2 pathway. Collectively, these results indicate that HO-1 reduces gut permeability by enhancing autophagy level in CSLD, the increased autophagy establishes a HO-1-p62-Nrf2 positive feedback loop to further improve gut-liver axis disruption. Therefore, our study confirms the critical role of autophagy in HO-1 ameliorating gut-liver axis injury during CSLD, highlighting HO-1 as a promising therapeutic target.

Exploring Coaching Leadership Behaviours in Strength and Conditioning Coaching: Preferences of NCAA Division I and II Collegiate Student-Athletes Based on Task Dependence

To explore collegiate student-athletes? preferences of coaching leadership behaviours in strength and conditioning (SC) coaching and evaluate differences between the preferences of coaching leadership behaviours based on task dependence. Method a total of 145 National Collegiate Athletic Association student-athletes (independent sports athletes = 48, interdependent sports athletes = 97), aged 18-25 years, with a mean = 3 (SD = ±1) SC sessions per week, participated. Participants completed an electronic questionnaire involving the athletes? preference version of the Revised Leadership Scale for SC. Results summary statistics revealed that the most favoured behaviour was 'training and instruction', median = 4.5 (IQR = 1.0), while the least favoured was ?autocratic?, median = 2.0 (IQR = 0.5). Independent sports athletes preferred training and instruction more (median = 5.0, IQR = 0.6) than interdependent sports athletes (median = 4.5, IQR = 1.0). Interdependent sports athletes preferred social support more (median = 3.5, IQR = 1.0) than independent sports athletes (median = 3.0, IQR = 0.6). The observation of a marginal statistically significant difference for social support suggests task-dependence-based variations (p = 0.018). However, small effect sizes indicate that differences are not practically significant. Conclusion and recommendation: this study presents insights into the preferences of coaching leadership behaviours among student-athletes in SC coaching. It highlights key behaviours such as training and instruction, positive feedback, situational considerations, and social support. These findings inform coaching practice and provide a foundation for further research into coaching leadership behaviours in SC coaching.

Master resilience trainer course quality improvement evaluation

ABSTRACT The Master Resilience Training (MRT) course is the U.S. Army’s resilience program of record to develop soldiers as resilience trainers within their home units. The Walter Reed Army Institute of Research (WRAIR) recently conducted an MRT Quality Improvement Evaluation (QIE) to understand perceptions of the MRT course, determine the effectiveness of the course, and provide revision recommendations for the next iteration of the course. Soldiers who were completing the MRT course were invited to take surveys and participate in interviews. Results from quantitative and qualitative data suggest that most participants felt satisfied with the training and that it was relevant for soldiers. Most participants also agreed that the training would help them become better soldiers and leaders. Along with positive feedback about the course, participants also identified areas that could be improved. Soldier feedback along with program evaluators’ observation informed recommendations for improving the overall MRT course and its implementation. Program evaluators recommend MRT participants learn fewer and less complex skills, focus on coach education throughout the course, and highlight leader development by promoting motivation and enhancing effective communication. Program evaluators recommendations for ways to improve buy-in from leaders and graduated MRTs are also discussed.

Study on the regulatory mechanism of latent membrane protein 2A on GCNT3 expression in nasopharyngeal carcinoma.

O-Glycan synthesis enzyme glucosaminyl (N-acetyl) transferase 3 (GCNT3) is closely related to the occurrence and development of various cancers.However, the regulatory mechanism and function of GCNT3 in nasopharyngeal carcinoma (NPC) are still poorly understood. This study aims to explore the regulatory mechanism of EBV-encoded latent membrane protein 2A (LMP2A) on GCNT3 and the biological role of GCNT3 in NPC.The results show that LMP2A can activate GCNT3 through the mTORC1 pathway, and there is a positive feedback between the mTORC1 and GCNT3. GCNT3 regulates EMT progression by forming a complex with ZEB1 to promote cell migration. GCNT3 can also promote cell proliferation. These findings indicate that targeting the LMP2A-mTORC1-GCNT3 axis may represent a novel therapeutic target in NPC.

PI15, a novel secreted WNT-signaling antagonist, regulates chondrocyte differentiation

ABSTRACT Purpose/Aim of Study During the development of the vertebrate skeleton, the progressive differentiation and maturation of chondrocytes from mesenchymal progenitors is precisely coordinated by multiple secreted factors and signaling pathways. The WNT signaling pathway has been demonstrated to play a major role in chondrogenesis. However, the identification of secreted factors that fine-tune WNT activity has remained elusive. Here, in this study, we have identified PI15 (peptidase inhibitor 15, protease Inhibitor 15, SugarCrisp), a member of the CAP (cysteine rich secretory proteins, antigen 5, and pathogenesis related 1 proteins) protein superfamily, as a novel secreted WNT antagonist dynamically upregulated during chondrocyte differentiation. Materials and Methods ATDC5 cells, C3H10T1/2 micromass cultures and primary chondrocyte cells were used as in vitro models of chondrogenesis. PI15 levels were stably depleted or overexpressed by viral shRNA or expression vectors. Chondrogenesis was evaluated by qPCR gene expression analysis and Alcian blue staining. Protein interactions were determined by coimmunoprecipitation assays. Results and Conclusions shRNA-mediated knockdown of PI15 in ATDC5 cells, C3H10T1/2 cells or primary chondrocytes inhibits chondrogenesis, whereas the overexpression of PI15 strongly enhances chondrogenic potential. Mechanistically, PI15 binds to the LRP6 WNT co-receptor and blocks WNT-induced LRP6 phosphorylation, thus repressing WNT-induced transcriptional activity and alleviating the inhibitory effect of WNT signaling on chondrogenesis. Altogether, our findings suggest that PI15 acts as a key regulator of chondrogenesis and unveils a mechanism through which chondrocyte-derived molecules can modulate WNT activity as differentiation proceeds, thereby creating a positive feedback loop that further drives differentiation.

More Than a Game: Building a Workplace Culture of Positivity and Belonging Among Nurses.

This performance improvement project introduced a mobile social gaming app for sharing positive feedback from patients and coworkers with nurses. After deployment, the app supported creating improvements as a supportive structure for engaging nurses in peer recognition to build a workplace culture of positivity and belonging. The authors discuss findings from the app's adoption and use among nurses and offer recommendations for leaders.

What do patients and families observe aboutpediatric safety?: A thematic analysis of real-time narratives.

Data on inpatient safety are documented by hospital staff through incident reporting (IR) systems. Safety observations from families or patients are rarely captured. The Family Input for Quality and Safety (FIQS) study created a mobile health tool for pediatric patients and their families to anonymously report safety observations in real time during hospitalization. The study objectives were to describe these observations and identify domains salient to safety. In this observational study, we analyzed pediatric patient safety reports from June 2017 to April 2018. Participants were: English-speaking family membersand hospitalized patients ≥13 years old. The analysis had two stages: 1) assessment of whether narratives met established safety event criteria and whether there were companion IRs; 2) thematic analysis to identify domains. Of 248 enrolled participants, 58 submitted 120 narrative reports. Of the narratives, 68 (57%) met safety event criteria, while only one (0.8%) corresponded to a staff-reported IR. 25% of narratives shared positive feedback about patient safety efforts; 75% shared constructive feedback. We identified domains particularly salient to safety:1) patients and families as safety actors; 2) emotional safety; 3) system-centered care; and 4) shared safety domains, including medication, communication, and environment of care. Some domains capture data that is otherwise difficult to obtain (#1-3), while others fit within standard healthcare safety domains (#4). Patients and families observe and report salient safety events that can fill gaps in IR data. Healthcare leaders should consider incorporating patient and family observations-collected with an option for anonymity and eliciting both positive and constructive comments.

Improving the undergraduate Science experience through an evidence-based framework for design, implementation and evaluation of flipped learning

Flipped Learning (FL) is a student-centred pedagogical approach where new content is introduced prior to class which permits more time during class for active learning. Despite the growing body of evidence of the effectiveness of FL, many educators are reluctant to adopt this approach to teaching or are unsure of how to implement FL in their classes. Many students are uncertain of how to adapt their approaches to learning to a FL curriculum. In response to these challenges and calls for a robust framework to guide the design and implementation of FL, we developed the Flipped Teacher and Flipped Learner (FTFL) Framework based on the pedagogical literature. This paper reports on the use of our FTFL framework in the redesign of a large first year science subject from a traditional delivery to a FL delivery. We evaluated the efficacy of the redesign using a mixed methods approach with data on students’ interactions with FL activities, and student and educator experiences. Findings from two iterations of the redesign indicate successful implementation of FL through high student engagement with online and class materials, and positive feedback from students and academics. Using the FTFL framework to guide the design and integration of FL, with an emphasis on clear communication, is key to our successful FL intervention and support of student learning.

Phosphorylation of FOXK2 at Thr13 and Ser30 by PDK2 sustains glycolysis through a positive feedback manner in ovarian cancer.

Ovarian cancer is one of the most common gynecological malignant tumors with insidious onset, strong invasiveness, and poor prognosis. Metabolic alteration, particularly aerobic glycolysis, which is tightly regulated by transcription factors, is associated with the malignant behavior of OC. We screened FOXK2 in this study as a key transcription factor that regulates glycolysis in OC. FOXK2 is overly expressed in OC, and poor prognosis is predicted by overexpression. FOXK2 promotes OC cell proliferation both in vitro and in vivo and cell migration in vitro. Further studies showed that PDK2 directly binds to the forkhead-associated (FHA) domain of FOXK2 to phosphorylate FOXK2 at Thr13 and Ser30, thereby enhancing the transcriptional activity of FOXK2. FOXK2 transcriptionally regulates the expression of PDK2, thus forming positive feedback to sustain glycolysis in OC cells.

Coralline Algal Population Explosion in an Overgrazed Seagrass Meadow: Conditional Outcomes of Intraspecific and Interspecific Interactions

AbstractInteractions such as mutualism and facilitation are common in ecosystems established by foundation species; however, their outcomes vary and show conditionality. In a Mexican Caribbean Bay, a seagrass-coralline algae (rhodoliths) mutualism protects the seagrass Thalassia testudinum from green turtle overgrazing. We postulate that the state of the seagrass meadow in this bay depends on the strengths of the interactions among seagrasses, green turtles, and coralline algae. Spatio-temporal changes through satellite imagery showed rhodolith bed developed rapidly from 2009 (undetected) to 2016 (bed of 6934 m2). Typically, such rapid expansion of the rhodoliths does not occur in seagrass meadows. An in situ growth experiment of coralline algae showed that a combination of reduction in light and wave movement (usual in dense seagrass meadows) significantly reduced their growth rates. In the rhodolith beds, the growth rates of the coralline algae Neogoniolithon sp. and Amphiroa sp. were high at 9.5 mm and 15.5 mm per growth tip y−1, respectively. In a second experiment, we found lower mortality in coralline algae within a rhodolith bed compared to algae placed outside the bed, likely explained by the reduced resuspension that we found in a third experiment, and this positive feedback may explain the high population increase in the rhodoliths, once established when the turtles grazed down the seagrass canopy. Therefore, the grazing-protection mutualism between seagrasses and coralline algae is thus conditional and came into existence under a co-occurrence of intensive grazing pressure and rapid population growth of coralline algae facilitated by positive feedback from increased growth and reduced sediment resuspension by the dense rhodolith bed.

Indium Tin Oxide Induced Internal Positive Feedback and Indium Ion Transport in Perovskite Solar Cells.

Stability is the most pressing challenge hindering the commercialization of perovskite solar cells (PSCs), and previous efforts focused more on enhancing the resistance of PSCs to external stimulus. Here, we found that the indium tin oxide (ITO) will deteriorate the photovoltaic performance of PSCs through positive feedback cycles. Specifically, the perovskite degradation products will cross the electron transport layer to chemically etch the electrode ITO to generate In3+, which will migrate upwards into the perovskite film. Then, the reaction that corrodes ITO consumes the decomposition products of perovskite and shifts the balance of the perovskite decomposition reaction, further promoting the degradation and thus falling into a positive feedback cycle. Moreover, the In3+ in the perovskite film was found to accumulate at the upper surface, which would lead to n-type doping of perovskite film to form the energy barrier for interface carrier extraction. Subsequently, the chelating molecule ethylenediaminetetraacetic acid disodium salt (EDTA-2Na) was introduced onto ITO to firmly chelate the In3+ and prevent it from migrating upward, thus breaking this internal positive feedback cycle and significantly enhancing the efficiency and stability of PSCs. This work provides new perspectives for understanding the mechanism of photovoltaic performance loss and ionic transport in PSCs.

Patients as teachers: a within-subjects randomized pilot experiment of patient-led online learning modules for health professionals

PurposeMany health professions education programs involve people with lived experience as expert speakers. Such presentations may help learners better understand the realities of living with chronic illness or experiencing an acute health problem. However, lectures from only one or a small number of people may not adequately illustrate the perspectives and experiences of a diverse patient cohort. Additionally, logistical constraints such as public health restrictions or travel barriers may impede in-person presentations, particularly among people who have more restrictions on their time. Health professions education programs may benefit from understanding the potential effects of online patient-led presentations with a diverse set of speakers. We aimed to explore whether patient-led online learning modules about diabetes care would influence learners’ responses to clinical scenarios and to collect learners’ feedback about the modules.MethodThis within-subjects randomized experiment involved 26 third-year medical students at Université Laval in Quebec, Canada. Participation in the experiment was an optional component within a required course. Prior to the intervention, participating learners responded to three clinical scenarios randomly selected from a set of six such scenarios. Each participant responded to the other three scenarios after the intervention. The intervention consisted of patient-led online learning modules incorporating segments of narratives from 21 patient partners (11 racialized or Indigenous) describing why and how clinicians could provide patient-centered care. Working with clinical teachers and psychometric experts, we developed a scoring grid based on the biopsychosocial model and set 0.6 as a passing score. Independent evaluators, blinded to whether each response was collected before or after the intervention, then scored learners’ responses to scenarios using the grid. We used Fisher’s Exact test to compare proportions of passing scores before and after the intervention.ResultsLearners’ overall percentage of passing scores prior to the intervention was 66%. Following the intervention, the percentage of passing scores was 76% (p = 0.002). Overall, learners expressed appreciation and other positive feedback regarding the patient-led online learning modules.DiscussionFindings from this experiment suggest that learners can learn to provide better patient-centered care by watching patient-led online learning modules created in collaboration with a diversity of patient partners.

EXPERIENCES OF PACIFIC PATIENTS SEEKING ARTHROPLASTY IN NEW ZEALAND

Pacific people in New Zealand experience significant disparity in health outcomes. There is little known about the burden of arthritis within this community or difficulties accessing specialist orthopaedic care. This qualitative study evaluated the experiences of Pacific patients who underwent hip or knee arthroplasty with a goal to identify barriers to accessing arthroplasty for this community.We interviewed Pacific patients within the Bay of Plenty region who had received either elective hip or knee arthroplasty between 2013 and 2022. Interviews were centred on perceptions of arthritis severity, duration of symptoms, primary care and specialist interactions. Patients were encouraged to offer feedback on ways to improve this experience.We identified 6087 publicly funded primary joints performed in Tauranga hospital and 58 patients were of Pacific ethnicity. After exclusion criteria was applied, we successfully interviewed 20 patients eligible for our study. Pacific patients represented 2.9% of the of the BOP catchment but only received 0.43% of the publicly funded joints. Most reported reluctance to seek help from primary care until symptoms were present for at least a year. Most commonly cited reasons for not seeking help were fear of hospital services and lack of awareness in the community about osteoarthritis.We identified a lack of community awareness of osteoarthritis and arthroplasty among Pacific. This may result in delayed presentation to primary care and decreased utilisation of publicly funded joint surgery. It is reassuring that most patients of Pacific ethnicity who receive primary hip or knee arthroplasty report a positive experience. Public health initiatives together with positive feedback from Pacific patients who have undergone surgery will help to increase awareness of arthroplasty as an option to restore function and relieve pain.

MyCare Journal

Apple ward in Hinchingbrooke Hospital consists of both medical andstroke patients. Many patients on the unit have had ailments which affecttheir communication, which ranges from dementia to dysarthria followingstrokes. The aim of this project was to provide a tool that would helpstaff be more familiar with patients who may struggle to communicateand provide more comfort in their care, for example by playing TV showsthey enjoy, or providing food and beverages they enjoy, which wouldmake their stay in hospital more pleasant. To develop a communicationtool which would enable the staff on stroke/geriatrics ward atHinchingbrooke hospital to provide personalised care for patients whomay struggle to communicate. The leaflet was based off ‘Patient Passports’which were used in the critical care unit in Salford Royal hospital. TheMyCare journals are to be filled by family/friends and left at the bedsidefor staff to access when needed.I developed the MyCare journal on Canva and I used bright colours todraw attention from the audience. I took inspiration from the questions on the original Patient Passport but tailored them to make them moresuitable for the geriatrics/stroke ward. I used semi-structured interviewsto assess feedback from relatives and staff and provided them with copiesto keep and use. The questions included their view on the design,prompts, and whether they would change anything with the MyCarejournal. Overall, I interviewed three patient families, two doctors on themedical team, and one stroke occupational therapist.This is an ongoing pilot project, so I am continuing to gain feedback.So far, there has been overwhelming positive feedback from families,they have liked the purpose of the leaflet and the design. They alsofound the section on mobility aids and glasses extremely useful. Thefeedback from the medical and therapy team has also been very positive,they felt that it would help to personalise patient care and makethe patients more comfortable during their stay. Overall, families havegiven positive feedback on the MyCare journal. One challenge I havefaced is how to encourage use of the passport on the ward as this wouldrequire ongoing prompts to ensure it is given to patients with communicationdifficulties. There was quite a limited sample size, partially dueto time constraints and partially due to the fact that not all familieswere available. There is also difficulty in implementing and keeping theproject going. I discussed this with the occupational therapy team; theypreviously had a similar project ongoing, and they were keen to use thisone with patients.

Indium Tin Oxide Induced Internal Positive Feedback and Indium Ion Transport in Perovskite Solar Cells

Stability is the most pressing challenge hindering the commercialization of perovskite solar cells (PSCs), and previous efforts focused more on enhancing the resistance of PSCs to external stimulus. Here, we found that the indium tin oxide (ITO) will deteriorate the photovoltaic performance of PSCs through positive feedback cycles. Specifically, the perovskite degradation products will cross the electron transport layer to chemically etch the electrode ITO to generate In3+, which will migrate upwards into the perovskite film. Then, the reaction that corrodes ITO consumes the decomposition products of perovskite and shifts the balance of the perovskite decomposition reaction, further promoting the degradation and thus falling into a positive feedback cycle. Moreover, the In3+ in the perovskite film was found to accumulate at the upper surface, which would lead to n‐type doping of perovskite film to form the energy barrier for interface carrier extraction. Subsequently, the chelating molecule ethylenediaminetetraacetic acid disodium salt (EDTA‐2Na) was introduced onto ITO to firmly chelate the In3+ and prevent it from migrating upward, thus breaking this internal positive feedback cycle and significantly enhancing the efficiency and stability of PSCs. This work provides new perspectives for understanding the mechanism of photovoltaic performance loss and ionic transport in PSCs.

Blood flow regulates acvrl1 transcription via ligand-dependent Alk1 activity.

Hereditary hemorrhagic telangiectasia (HHT) is an autosomal dominant disease characterized by the development of arteriovenous malformations (AVMs) that can result in significant morbidity and mortality. HHT is caused primarily by mutations in bone morphogenetic protein receptors ACVRL1/ALK1, a signaling receptor, or endoglin (ENG), an accessory receptor. Because overexpression of Acvrl1 prevents AVM development in both Acvrl1 and Eng null mice, enhancing ACVRL1 expression may be a promising approach to development of targeted therapies for HHT. Therefore, we sought to understand the molecular mechanism of ACVRL1 regulation. We previously demonstrated in zebrafish embryos that acvrl1 is predominantly expressed in arterial endothelial cells and that expression requires blood flow. Here, we document that flow dependence exhibits regional heterogeneity and that acvrl1 expression is rapidly restored after reinitiation of flow. Furthermore, we find that acvrl1 expression is significantly decreased in mutants that lack the circulating Alk1 ligand, Bmp10, and that, in the absence of flow, intravascular injection of BMP10 or the related ligand, BMP9, restores acvrl1 expression in an Alk1-dependent manner. Using a transgenic acvrl1:egfp reporter line, we find that flow and Bmp10 regulate acvrl1 at the level of transcription. Finally, we observe similar ALK1 ligand-dependent increases in ACVRL1 in human endothelial cells subjected to shear stress. These data suggest that ligand-dependent Alk1 activity acts downstream of blood flow to maintain or enhance acvrl1 expression via a positive feedback mechanism, and that ALK1 activating therapeutics may have dual functionality by increasing both ALK1 signaling flux and ACVRL1 expression.

Exploring the Impact of Media Content on Social Media Engagement Through Comment Analysis

This study investigates audience participation on the social media platforms of Kantipur Daily, Sagarmatha Radio, and Nepal Television using survey data collected in 2024. A review of comments on Facebook and X(Twitter) indicated that positive feedback is primarily focused on sports and social/health/lifestyle news Moreover, opinions and economic news receive relatively less attention than political news. The study highlights particular areas that need more research and offers insightful information about audience dynamics in the context of digital communication. This finding emphasizes the value of social media as a political communication tool, which is especially clear from the positive comments on Political News. Additionally, despite certain restrictions, the results show how the communication landscape is changing and how social media is improving news accessibility and content quality. The finding indicates that social media has become a new arena for public affairs, and its results show that Facebook is more widely used than X.